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labels cone photoreceptors  (Vector Laboratories)


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    Vector Laboratories labels cone photoreceptors
    Labels Cone Photoreceptors, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 385 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cone+photoreceptors/Fluorescein+labeled+Peanut+Agglutinin+(PNA)/pmc04278841-3-10-18
    Average 95 stars, based on 385 article reviews
    labels cone photoreceptors - by Bioz Stars, 2026-09
    95/100 stars

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    other:

    Article Title: The transcription factor RBP-J is essential for retinal cell differentiation and lamination
    Article Snippet: For detection of cone photoreceptors, fluorescein-conjugated peanut agglutinin (PNA; 1:200; Vector) was employed.

    Article Title: Dose-related changes in retinal function and PKC-alpha expression in rabbits on vigabatrin medication. Effect of vigabatrin in the rabbit eye.
    Article Snippet: Background To investigate, in a rabbit model, the effect of two different doses of vigabatrin (VGB) on retinal function and morphology.. Methods Twenty-nine rabbits of mixed strain were divided into two groups, receiving either high-dose (n=15) or lowdose (n=14) oral VGB treatment (cumulative dose 29.8± 2.9 g and 14.2±0.6 g respectively).. Ten rabbits receiving water served as control animals.

    Labeling:

    Article Title: Remodeling of the Retina Following Photoreceptor Degeneration That Enables Targeted Azobenzene Photosensitization
    Article Snippet: Microglia and infiltrating macrophages were labeled with rabbit anti-ionized calcium binding adaptor molecule 1 (IbA1, 1:1500; Cat. No# 019-19741; Wako Pure Chemical Industries, VA, USA). .. Cone photoreceptors were labeled with rhodamine-labeled peanut agglutinin (PNA, 1:2500; Vector Laboratories, Burlingame, CA). .. Sections were rinsed 3 times in PB and were incubated with secondary antibody: goat anti-mouse, or goat anti-rabbit conjugated to AlexaFluor®488 or AlexaFluor® 594 (Thermofisher Scientific, VIC, Australia; diluted 1:500) as required for 90 minutes.



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    labels cone photoreceptors  (Vector Laboratories)
    95
    Vector Laboratories labels cone photoreceptors
    Labels Cone Photoreceptors, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cone+photoreceptors/Fluorescein+labeled+Peanut+Agglutinin+(PNA)/pmc04278841-3-10-18
    Average 95 stars, based on 1 article reviews
    labels cone photoreceptors - by Bioz Stars, 2026-09
    95/100 stars
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    cone photoreceptor terminals  (Oxford Instruments)
    99
    Oxford Instruments cone photoreceptor terminals
    Postnatal changes in EPSCs recorded from HCs and in HC and cone <t>photoreceptor</t> synaptic terminal connectivity. ( A ) Representative EPSC traces at different developmental stages. HCs were held at a V h of −60 mV. ( B ) Zoomed in view of tonic EPSCs recorded from a matured HC. Asterisks indicate EPSC events. ( C ) The mean frequency of tonic EPSCs. Significance was determined by one-way ANOVA test ( P < 0.001, n = 8 cells). Tukey's multiple comparisons test (>P30 vs. P6–P7: P < 0.001, >P30 vs. P8–P9: P = 0.01). ( D ) Mean amplitude of tonic EPSCs. Significance was determined by one-way ANOVA test ( P = 0.953, n = 8 cells). ( E ) The mean charge transfer rate of tonic EPSCs (−pC/s). Significance was determined by the Kruskal–Wallis test ( P = 0.0077, n = 8 cells). Post hoc Mann–Whitney U test (>P30 vs. P6–P7: P = 0.023). ( F ) Immunofluorescence staining of horizontal retinal slices with labelling of individual HCs loaded with neurobiotin ( red ) and EGFP-labelled cone photoreceptor terminals ( green ). Dashed yellow lines outline cone photoreceptor terminals. ( G ) The mean number of contacting cone photoreceptor terminals per HC. Significance was determined by one-way ANOVA test ( P = 0.0695, n = 6–9 cells). ( H ) The mean cone photoreceptor terminal area. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 142–149 terminals). Post hoc Mann–Whitney U test ( P < 0.001 for all age groups vs. >P30, P6–P7 vs. P8–P9: P < 0.001, P8–P9 vs. P10–P11: P < 0.001, P10–P11 vs. P12–P13: P = 0.0098). ( I ) The mean number of HC contacts at cone photoreceptor terminals. Significance was determined by Kruskal-Wallis test ( P < 0.001, n = 51–119 terminals). Post hoc Mann–Whitney U test (P6–P7 vs. P8–P 9: P < 0.001, >P30 vs. P6–P 7: P < 0.001, P10–P 11 vs. P12–P 13: P = 0.0037).
    Cone Photoreceptor Terminals, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cone photoreceptors  (Gallus BioPharmaceuticals)
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    Gallus BioPharmaceuticals cone photoreceptors
    Postnatal changes in EPSCs recorded from HCs and in HC and cone <t>photoreceptor</t> synaptic terminal connectivity. ( A ) Representative EPSC traces at different developmental stages. HCs were held at a V h of −60 mV. ( B ) Zoomed in view of tonic EPSCs recorded from a matured HC. Asterisks indicate EPSC events. ( C ) The mean frequency of tonic EPSCs. Significance was determined by one-way ANOVA test ( P < 0.001, n = 8 cells). Tukey's multiple comparisons test (>P30 vs. P6–P7: P < 0.001, >P30 vs. P8–P9: P = 0.01). ( D ) Mean amplitude of tonic EPSCs. Significance was determined by one-way ANOVA test ( P = 0.953, n = 8 cells). ( E ) The mean charge transfer rate of tonic EPSCs (−pC/s). Significance was determined by the Kruskal–Wallis test ( P = 0.0077, n = 8 cells). Post hoc Mann–Whitney U test (>P30 vs. P6–P7: P = 0.023). ( F ) Immunofluorescence staining of horizontal retinal slices with labelling of individual HCs loaded with neurobiotin ( red ) and EGFP-labelled cone photoreceptor terminals ( green ). Dashed yellow lines outline cone photoreceptor terminals. ( G ) The mean number of contacting cone photoreceptor terminals per HC. Significance was determined by one-way ANOVA test ( P = 0.0695, n = 6–9 cells). ( H ) The mean cone photoreceptor terminal area. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 142–149 terminals). Post hoc Mann–Whitney U test ( P < 0.001 for all age groups vs. >P30, P6–P7 vs. P8–P9: P < 0.001, P8–P9 vs. P10–P11: P < 0.001, P10–P11 vs. P12–P13: P = 0.0098). ( I ) The mean number of HC contacts at cone photoreceptor terminals. Significance was determined by Kruskal-Wallis test ( P < 0.001, n = 51–119 terminals). Post hoc Mann–Whitney U test (P6–P7 vs. P8–P 9: P < 0.001, >P30 vs. P6–P 7: P < 0.001, P10–P 11 vs. P12–P 13: P = 0.0037).
    Cone Photoreceptors, supplied by Gallus BioPharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti-cone arrestin - arrestin 3/cone photoreceptors – rabbit  (Millipore)
    90
    Millipore anti-cone arrestin - arrestin 3/cone photoreceptors – rabbit
    Postnatal changes in EPSCs recorded from HCs and in HC and cone <t>photoreceptor</t> synaptic terminal connectivity. ( A ) Representative EPSC traces at different developmental stages. HCs were held at a V h of −60 mV. ( B ) Zoomed in view of tonic EPSCs recorded from a matured HC. Asterisks indicate EPSC events. ( C ) The mean frequency of tonic EPSCs. Significance was determined by one-way ANOVA test ( P < 0.001, n = 8 cells). Tukey's multiple comparisons test (>P30 vs. P6–P7: P < 0.001, >P30 vs. P8–P9: P = 0.01). ( D ) Mean amplitude of tonic EPSCs. Significance was determined by one-way ANOVA test ( P = 0.953, n = 8 cells). ( E ) The mean charge transfer rate of tonic EPSCs (−pC/s). Significance was determined by the Kruskal–Wallis test ( P = 0.0077, n = 8 cells). Post hoc Mann–Whitney U test (>P30 vs. P6–P7: P = 0.023). ( F ) Immunofluorescence staining of horizontal retinal slices with labelling of individual HCs loaded with neurobiotin ( red ) and EGFP-labelled cone photoreceptor terminals ( green ). Dashed yellow lines outline cone photoreceptor terminals. ( G ) The mean number of contacting cone photoreceptor terminals per HC. Significance was determined by one-way ANOVA test ( P = 0.0695, n = 6–9 cells). ( H ) The mean cone photoreceptor terminal area. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 142–149 terminals). Post hoc Mann–Whitney U test ( P < 0.001 for all age groups vs. >P30, P6–P7 vs. P8–P9: P < 0.001, P8–P9 vs. P10–P11: P < 0.001, P10–P11 vs. P12–P13: P = 0.0098). ( I ) The mean number of HC contacts at cone photoreceptor terminals. Significance was determined by Kruskal-Wallis test ( P < 0.001, n = 51–119 terminals). Post hoc Mann–Whitney U test (P6–P7 vs. P8–P 9: P < 0.001, >P30 vs. P6–P 7: P < 0.001, P10–P 11 vs. P12–P 13: P = 0.0037).
    Anti Cone Arrestin Arrestin 3/Cone Photoreceptors – Rabbit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cone+photoreceptors/anti+cone+arrestin+++arrestin+3+cone+photoreceptors+++rabbit/pmc11460503-3-0-11
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    automated cone photoreceptor cell segmentation and identification  (IEEE Access)
    90
    IEEE Access automated cone photoreceptor cell segmentation and identification
    Postnatal changes in EPSCs recorded from HCs and in HC and cone <t>photoreceptor</t> synaptic terminal connectivity. ( A ) Representative EPSC traces at different developmental stages. HCs were held at a V h of −60 mV. ( B ) Zoomed in view of tonic EPSCs recorded from a matured HC. Asterisks indicate EPSC events. ( C ) The mean frequency of tonic EPSCs. Significance was determined by one-way ANOVA test ( P < 0.001, n = 8 cells). Tukey's multiple comparisons test (>P30 vs. P6–P7: P < 0.001, >P30 vs. P8–P9: P = 0.01). ( D ) Mean amplitude of tonic EPSCs. Significance was determined by one-way ANOVA test ( P = 0.953, n = 8 cells). ( E ) The mean charge transfer rate of tonic EPSCs (−pC/s). Significance was determined by the Kruskal–Wallis test ( P = 0.0077, n = 8 cells). Post hoc Mann–Whitney U test (>P30 vs. P6–P7: P = 0.023). ( F ) Immunofluorescence staining of horizontal retinal slices with labelling of individual HCs loaded with neurobiotin ( red ) and EGFP-labelled cone photoreceptor terminals ( green ). Dashed yellow lines outline cone photoreceptor terminals. ( G ) The mean number of contacting cone photoreceptor terminals per HC. Significance was determined by one-way ANOVA test ( P = 0.0695, n = 6–9 cells). ( H ) The mean cone photoreceptor terminal area. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 142–149 terminals). Post hoc Mann–Whitney U test ( P < 0.001 for all age groups vs. >P30, P6–P7 vs. P8–P9: P < 0.001, P8–P9 vs. P10–P11: P < 0.001, P10–P11 vs. P12–P13: P = 0.0098). ( I ) The mean number of HC contacts at cone photoreceptor terminals. Significance was determined by Kruskal-Wallis test ( P < 0.001, n = 51–119 terminals). Post hoc Mann–Whitney U test (P6–P7 vs. P8–P 9: P < 0.001, >P30 vs. P6–P 7: P < 0.001, P10–P 11 vs. P12–P 13: P = 0.0037).
    Automated Cone Photoreceptor Cell Segmentation And Identification, supplied by IEEE Access, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cone+photoreceptors/automated+cone+photoreceptor+cell+segmentation+and+identification/pm36356384-253-10-29
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    cone photoreceptors  (Vector Laboratories)
    86
    Vector Laboratories cone photoreceptors
    Postnatal changes in EPSCs recorded from HCs and in HC and cone <t>photoreceptor</t> synaptic terminal connectivity. ( A ) Representative EPSC traces at different developmental stages. HCs were held at a V h of −60 mV. ( B ) Zoomed in view of tonic EPSCs recorded from a matured HC. Asterisks indicate EPSC events. ( C ) The mean frequency of tonic EPSCs. Significance was determined by one-way ANOVA test ( P < 0.001, n = 8 cells). Tukey's multiple comparisons test (>P30 vs. P6–P7: P < 0.001, >P30 vs. P8–P9: P = 0.01). ( D ) Mean amplitude of tonic EPSCs. Significance was determined by one-way ANOVA test ( P = 0.953, n = 8 cells). ( E ) The mean charge transfer rate of tonic EPSCs (−pC/s). Significance was determined by the Kruskal–Wallis test ( P = 0.0077, n = 8 cells). Post hoc Mann–Whitney U test (>P30 vs. P6–P7: P = 0.023). ( F ) Immunofluorescence staining of horizontal retinal slices with labelling of individual HCs loaded with neurobiotin ( red ) and EGFP-labelled cone photoreceptor terminals ( green ). Dashed yellow lines outline cone photoreceptor terminals. ( G ) The mean number of contacting cone photoreceptor terminals per HC. Significance was determined by one-way ANOVA test ( P = 0.0695, n = 6–9 cells). ( H ) The mean cone photoreceptor terminal area. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 142–149 terminals). Post hoc Mann–Whitney U test ( P < 0.001 for all age groups vs. >P30, P6–P7 vs. P8–P9: P < 0.001, P8–P9 vs. P10–P11: P < 0.001, P10–P11 vs. P12–P13: P = 0.0098). ( I ) The mean number of HC contacts at cone photoreceptor terminals. Significance was determined by Kruskal-Wallis test ( P < 0.001, n = 51–119 terminals). Post hoc Mann–Whitney U test (P6–P7 vs. P8–P 9: P < 0.001, >P30 vs. P6–P 7: P < 0.001, P10–P 11 vs. P12–P 13: P = 0.0037).
    Cone Photoreceptors, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nb100 1028 bop rabbit cone photoreceptors outer segments  (Novus Biologicals)
    97
    Novus Biologicals nb100 1028 bop rabbit cone photoreceptors outer segments
    Postnatal changes in EPSCs recorded from HCs and in HC and cone <t>photoreceptor</t> synaptic terminal connectivity. ( A ) Representative EPSC traces at different developmental stages. HCs were held at a V h of −60 mV. ( B ) Zoomed in view of tonic EPSCs recorded from a matured HC. Asterisks indicate EPSC events. ( C ) The mean frequency of tonic EPSCs. Significance was determined by one-way ANOVA test ( P < 0.001, n = 8 cells). Tukey's multiple comparisons test (>P30 vs. P6–P7: P < 0.001, >P30 vs. P8–P9: P = 0.01). ( D ) Mean amplitude of tonic EPSCs. Significance was determined by one-way ANOVA test ( P = 0.953, n = 8 cells). ( E ) The mean charge transfer rate of tonic EPSCs (−pC/s). Significance was determined by the Kruskal–Wallis test ( P = 0.0077, n = 8 cells). Post hoc Mann–Whitney U test (>P30 vs. P6–P7: P = 0.023). ( F ) Immunofluorescence staining of horizontal retinal slices with labelling of individual HCs loaded with neurobiotin ( red ) and EGFP-labelled cone photoreceptor terminals ( green ). Dashed yellow lines outline cone photoreceptor terminals. ( G ) The mean number of contacting cone photoreceptor terminals per HC. Significance was determined by one-way ANOVA test ( P = 0.0695, n = 6–9 cells). ( H ) The mean cone photoreceptor terminal area. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 142–149 terminals). Post hoc Mann–Whitney U test ( P < 0.001 for all age groups vs. >P30, P6–P7 vs. P8–P9: P < 0.001, P8–P9 vs. P10–P11: P < 0.001, P10–P11 vs. P12–P13: P = 0.0098). ( I ) The mean number of HC contacts at cone photoreceptor terminals. Significance was determined by Kruskal-Wallis test ( P < 0.001, n = 51–119 terminals). Post hoc Mann–Whitney U test (P6–P7 vs. P8–P 9: P < 0.001, >P30 vs. P6–P 7: P < 0.001, P10–P 11 vs. P12–P 13: P = 0.0037).
    Nb100 1028 Bop Rabbit Cone Photoreceptors Outer Segments, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cone photoreceptor function loss 5 mouse strain  (Jackson Laboratory)
    90
    Jackson Laboratory cone photoreceptor function loss 5 mouse strain
    Postnatal changes in EPSCs recorded from HCs and in HC and cone <t>photoreceptor</t> synaptic terminal connectivity. ( A ) Representative EPSC traces at different developmental stages. HCs were held at a V h of −60 mV. ( B ) Zoomed in view of tonic EPSCs recorded from a matured HC. Asterisks indicate EPSC events. ( C ) The mean frequency of tonic EPSCs. Significance was determined by one-way ANOVA test ( P < 0.001, n = 8 cells). Tukey's multiple comparisons test (>P30 vs. P6–P7: P < 0.001, >P30 vs. P8–P9: P = 0.01). ( D ) Mean amplitude of tonic EPSCs. Significance was determined by one-way ANOVA test ( P = 0.953, n = 8 cells). ( E ) The mean charge transfer rate of tonic EPSCs (−pC/s). Significance was determined by the Kruskal–Wallis test ( P = 0.0077, n = 8 cells). Post hoc Mann–Whitney U test (>P30 vs. P6–P7: P = 0.023). ( F ) Immunofluorescence staining of horizontal retinal slices with labelling of individual HCs loaded with neurobiotin ( red ) and EGFP-labelled cone photoreceptor terminals ( green ). Dashed yellow lines outline cone photoreceptor terminals. ( G ) The mean number of contacting cone photoreceptor terminals per HC. Significance was determined by one-way ANOVA test ( P = 0.0695, n = 6–9 cells). ( H ) The mean cone photoreceptor terminal area. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 142–149 terminals). Post hoc Mann–Whitney U test ( P < 0.001 for all age groups vs. >P30, P6–P7 vs. P8–P9: P < 0.001, P8–P9 vs. P10–P11: P < 0.001, P10–P11 vs. P12–P13: P = 0.0098). ( I ) The mean number of HC contacts at cone photoreceptor terminals. Significance was determined by Kruskal-Wallis test ( P < 0.001, n = 51–119 terminals). Post hoc Mann–Whitney U test (P6–P7 vs. P8–P 9: P < 0.001, >P30 vs. P6–P 7: P < 0.001, P10–P 11 vs. P12–P 13: P = 0.0037).
    Cone Photoreceptor Function Loss 5 Mouse Strain, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    red/green double cone photoreceptors  (Baier labs)
    90
    Baier labs red/green double cone photoreceptors
    Postnatal changes in EPSCs recorded from HCs and in HC and cone <t>photoreceptor</t> synaptic terminal connectivity. ( A ) Representative EPSC traces at different developmental stages. HCs were held at a V h of −60 mV. ( B ) Zoomed in view of tonic EPSCs recorded from a matured HC. Asterisks indicate EPSC events. ( C ) The mean frequency of tonic EPSCs. Significance was determined by one-way ANOVA test ( P < 0.001, n = 8 cells). Tukey's multiple comparisons test (>P30 vs. P6–P7: P < 0.001, >P30 vs. P8–P9: P = 0.01). ( D ) Mean amplitude of tonic EPSCs. Significance was determined by one-way ANOVA test ( P = 0.953, n = 8 cells). ( E ) The mean charge transfer rate of tonic EPSCs (−pC/s). Significance was determined by the Kruskal–Wallis test ( P = 0.0077, n = 8 cells). Post hoc Mann–Whitney U test (>P30 vs. P6–P7: P = 0.023). ( F ) Immunofluorescence staining of horizontal retinal slices with labelling of individual HCs loaded with neurobiotin ( red ) and EGFP-labelled cone photoreceptor terminals ( green ). Dashed yellow lines outline cone photoreceptor terminals. ( G ) The mean number of contacting cone photoreceptor terminals per HC. Significance was determined by one-way ANOVA test ( P = 0.0695, n = 6–9 cells). ( H ) The mean cone photoreceptor terminal area. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 142–149 terminals). Post hoc Mann–Whitney U test ( P < 0.001 for all age groups vs. >P30, P6–P7 vs. P8–P9: P < 0.001, P8–P9 vs. P10–P11: P < 0.001, P10–P11 vs. P12–P13: P = 0.0098). ( I ) The mean number of HC contacts at cone photoreceptor terminals. Significance was determined by Kruskal-Wallis test ( P < 0.001, n = 51–119 terminals). Post hoc Mann–Whitney U test (P6–P7 vs. P8–P 9: P < 0.001, >P30 vs. P6–P 7: P < 0.001, P10–P 11 vs. P12–P 13: P = 0.0037).
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    Image Search Results


    Postnatal changes in EPSCs recorded from HCs and in HC and cone photoreceptor synaptic terminal connectivity. ( A ) Representative EPSC traces at different developmental stages. HCs were held at a V h of −60 mV. ( B ) Zoomed in view of tonic EPSCs recorded from a matured HC. Asterisks indicate EPSC events. ( C ) The mean frequency of tonic EPSCs. Significance was determined by one-way ANOVA test ( P < 0.001, n = 8 cells). Tukey's multiple comparisons test (>P30 vs. P6–P7: P < 0.001, >P30 vs. P8–P9: P = 0.01). ( D ) Mean amplitude of tonic EPSCs. Significance was determined by one-way ANOVA test ( P = 0.953, n = 8 cells). ( E ) The mean charge transfer rate of tonic EPSCs (−pC/s). Significance was determined by the Kruskal–Wallis test ( P = 0.0077, n = 8 cells). Post hoc Mann–Whitney U test (>P30 vs. P6–P7: P = 0.023). ( F ) Immunofluorescence staining of horizontal retinal slices with labelling of individual HCs loaded with neurobiotin ( red ) and EGFP-labelled cone photoreceptor terminals ( green ). Dashed yellow lines outline cone photoreceptor terminals. ( G ) The mean number of contacting cone photoreceptor terminals per HC. Significance was determined by one-way ANOVA test ( P = 0.0695, n = 6–9 cells). ( H ) The mean cone photoreceptor terminal area. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 142–149 terminals). Post hoc Mann–Whitney U test ( P < 0.001 for all age groups vs. >P30, P6–P7 vs. P8–P9: P < 0.001, P8–P9 vs. P10–P11: P < 0.001, P10–P11 vs. P12–P13: P = 0.0098). ( I ) The mean number of HC contacts at cone photoreceptor terminals. Significance was determined by Kruskal-Wallis test ( P < 0.001, n = 51–119 terminals). Post hoc Mann–Whitney U test (P6–P7 vs. P8–P 9: P < 0.001, >P30 vs. P6–P 7: P < 0.001, P10–P 11 vs. P12–P 13: P = 0.0037).

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Functional and Structural Development of Mouse Cone Photoreceptor Ribbon Synapses

    doi: 10.1167/iovs.63.3.21

    Figure Lengend Snippet: Postnatal changes in EPSCs recorded from HCs and in HC and cone photoreceptor synaptic terminal connectivity. ( A ) Representative EPSC traces at different developmental stages. HCs were held at a V h of −60 mV. ( B ) Zoomed in view of tonic EPSCs recorded from a matured HC. Asterisks indicate EPSC events. ( C ) The mean frequency of tonic EPSCs. Significance was determined by one-way ANOVA test ( P < 0.001, n = 8 cells). Tukey's multiple comparisons test (>P30 vs. P6–P7: P < 0.001, >P30 vs. P8–P9: P = 0.01). ( D ) Mean amplitude of tonic EPSCs. Significance was determined by one-way ANOVA test ( P = 0.953, n = 8 cells). ( E ) The mean charge transfer rate of tonic EPSCs (−pC/s). Significance was determined by the Kruskal–Wallis test ( P = 0.0077, n = 8 cells). Post hoc Mann–Whitney U test (>P30 vs. P6–P7: P = 0.023). ( F ) Immunofluorescence staining of horizontal retinal slices with labelling of individual HCs loaded with neurobiotin ( red ) and EGFP-labelled cone photoreceptor terminals ( green ). Dashed yellow lines outline cone photoreceptor terminals. ( G ) The mean number of contacting cone photoreceptor terminals per HC. Significance was determined by one-way ANOVA test ( P = 0.0695, n = 6–9 cells). ( H ) The mean cone photoreceptor terminal area. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 142–149 terminals). Post hoc Mann–Whitney U test ( P < 0.001 for all age groups vs. >P30, P6–P7 vs. P8–P9: P < 0.001, P8–P9 vs. P10–P11: P < 0.001, P10–P11 vs. P12–P13: P = 0.0098). ( I ) The mean number of HC contacts at cone photoreceptor terminals. Significance was determined by Kruskal-Wallis test ( P < 0.001, n = 51–119 terminals). Post hoc Mann–Whitney U test (P6–P7 vs. P8–P 9: P < 0.001, >P30 vs. P6–P 7: P < 0.001, P10–P 11 vs. P12–P 13: P = 0.0037).

    Article Snippet: Cone photoreceptor terminals were then 3D reconstructed in Imaris (BitPlane, Zurich, Switzerland), and ribbon or α1f labelling outside of the reconstructed cone terminal was subtracted.

    Techniques: MANN-WHITNEY, Immunofluorescence, Staining

    Postnatal changes in cone photoreceptor I Ca . ( A ) Example cone photoreceptor I Ca at the different developmental stages during a voltage ramp protocol from −80 mV to +40 mV, with a speed of 0.1875 mV/ms. ( B ) The mean amplitude of I Ca . Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 5–12 cells). Post hoc Mann–Whitney U tests (>P30 vs. P6–P7: P = 0.0011, >P30 vs. P8–P9: P < 0.001, >P30 vs. P10–P11: P < 0.001, P6–P7 vs. P8–P9: P = 0.0475, P8–P9 vs. P10–P11: P = 0.0292). ( C ) The mean half-maximal activation of the I Ca (V 50 ). Significance was determined by the one-way ANOVA test ( P < 0.001, n = 5–12 cells). Tukey's test for multiple comparisons (P6–P7 vs. P8–P9: P < 0.001, >P30 vs. P6–P7: P < 0.001, >P30 vs. P8–P9: P < 0.001, >P30 vs. P10–P11: P = 0.0024, >P30 vs. P12–P13: P < 0.001). ( D ) The mean I Ca slope. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 5–12 cells). Post hoc Mann–Whitney U tests (P6–P7 vs. P8–P9: P = 0.0032, >P30 vs. P6–P7: P = 0.0244, >P30 vs. P8–P9: P = 0.0032).

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Functional and Structural Development of Mouse Cone Photoreceptor Ribbon Synapses

    doi: 10.1167/iovs.63.3.21

    Figure Lengend Snippet: Postnatal changes in cone photoreceptor I Ca . ( A ) Example cone photoreceptor I Ca at the different developmental stages during a voltage ramp protocol from −80 mV to +40 mV, with a speed of 0.1875 mV/ms. ( B ) The mean amplitude of I Ca . Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 5–12 cells). Post hoc Mann–Whitney U tests (>P30 vs. P6–P7: P = 0.0011, >P30 vs. P8–P9: P < 0.001, >P30 vs. P10–P11: P < 0.001, P6–P7 vs. P8–P9: P = 0.0475, P8–P9 vs. P10–P11: P = 0.0292). ( C ) The mean half-maximal activation of the I Ca (V 50 ). Significance was determined by the one-way ANOVA test ( P < 0.001, n = 5–12 cells). Tukey's test for multiple comparisons (P6–P7 vs. P8–P9: P < 0.001, >P30 vs. P6–P7: P < 0.001, >P30 vs. P8–P9: P < 0.001, >P30 vs. P10–P11: P = 0.0024, >P30 vs. P12–P13: P < 0.001). ( D ) The mean I Ca slope. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 5–12 cells). Post hoc Mann–Whitney U tests (P6–P7 vs. P8–P9: P = 0.0032, >P30 vs. P6–P7: P = 0.0244, >P30 vs. P8–P9: P = 0.0032).

    Article Snippet: Cone photoreceptor terminals were then 3D reconstructed in Imaris (BitPlane, Zurich, Switzerland), and ribbon or α1f labelling outside of the reconstructed cone terminal was subtracted.

    Techniques: MANN-WHITNEY, Activation Assay

    Postnatal changes in AZ and synaptic ribbon architecture in cone photoreceptor synaptic terminals. ( A , B ) Immunofluorescence staining of horizontal retinal slices, labelling for cone photoreceptor terminals ( green ) and ( A ) the α1f subunit of Ca v 1.4 voltage-gated Ca 2+ channels ( red ) or ( B ) synaptic ribbons (ribeye A domain, red ), at the different developmental stages. Dashed yellow lines represent the border of individual cone photoreceptor terminals. ( C ) The mean number of AZs. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 30–38 terminals). Post hoc Mann–Whitney U tests ( P < 0.001 for P30 vs. all other age groups, P6 vs. P8, P = 0.0056, P8 vs. P10: P < 0.001). ( D ) The mean AZ area per cone photoreceptor terminal. Significance was determined by one-way ANOVA test ( P < 0.001, n = 30–38 terminals). Tukey's tests for multiple comparisons ( P < 0.001 for P30 vs. all other age groups, P6 vs. P8: P = 0.004, P8 vs. P10: P = 0.044). ( E ) Mean number of synaptic ribbons per cone photoreceptor terminal. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 29–34 terminals). Post hoc Mann–Whitney U tests ( P < 0.001 for P30 vs. all other age groups, P8 vs. P10: P = 0.0095). ( F ) The mean length of the synaptic ribbons. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 164–360 ribbons). Post hoc Mann–Whitney U tests ( P < 0.001 for P30 vs. all other age groups, P8 vs. P10: P < 0.001).

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Functional and Structural Development of Mouse Cone Photoreceptor Ribbon Synapses

    doi: 10.1167/iovs.63.3.21

    Figure Lengend Snippet: Postnatal changes in AZ and synaptic ribbon architecture in cone photoreceptor synaptic terminals. ( A , B ) Immunofluorescence staining of horizontal retinal slices, labelling for cone photoreceptor terminals ( green ) and ( A ) the α1f subunit of Ca v 1.4 voltage-gated Ca 2+ channels ( red ) or ( B ) synaptic ribbons (ribeye A domain, red ), at the different developmental stages. Dashed yellow lines represent the border of individual cone photoreceptor terminals. ( C ) The mean number of AZs. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 30–38 terminals). Post hoc Mann–Whitney U tests ( P < 0.001 for P30 vs. all other age groups, P6 vs. P8, P = 0.0056, P8 vs. P10: P < 0.001). ( D ) The mean AZ area per cone photoreceptor terminal. Significance was determined by one-way ANOVA test ( P < 0.001, n = 30–38 terminals). Tukey's tests for multiple comparisons ( P < 0.001 for P30 vs. all other age groups, P6 vs. P8: P = 0.004, P8 vs. P10: P = 0.044). ( E ) Mean number of synaptic ribbons per cone photoreceptor terminal. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 29–34 terminals). Post hoc Mann–Whitney U tests ( P < 0.001 for P30 vs. all other age groups, P8 vs. P10: P = 0.0095). ( F ) The mean length of the synaptic ribbons. Significance was determined by the Kruskal–Wallis test ( P < 0.001, n = 164–360 ribbons). Post hoc Mann–Whitney U tests ( P < 0.001 for P30 vs. all other age groups, P8 vs. P10: P < 0.001).

    Article Snippet: Cone photoreceptor terminals were then 3D reconstructed in Imaris (BitPlane, Zurich, Switzerland), and ribbon or α1f labelling outside of the reconstructed cone terminal was subtracted.

    Techniques: Immunofluorescence, Staining, MANN-WHITNEY

    Ultrastructural analysis of synaptic ribbon development. ( A ) Representative electron micrographs of cone photoreceptor terminals at the different developmental stages. Yellow arrows indicate attached synaptic ribbons, red arrows indicate free-floating synaptic ribbons. (–) Mean percentage of attached synaptic ribbons. Significance was determined by one-way ANOVA test ( P < 0.001, n = 3–4 animals). Post hoc Tukey's tests for multiple comparisons ( P < 0.001 for P30 vs. all other age groups).

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Functional and Structural Development of Mouse Cone Photoreceptor Ribbon Synapses

    doi: 10.1167/iovs.63.3.21

    Figure Lengend Snippet: Ultrastructural analysis of synaptic ribbon development. ( A ) Representative electron micrographs of cone photoreceptor terminals at the different developmental stages. Yellow arrows indicate attached synaptic ribbons, red arrows indicate free-floating synaptic ribbons. (–) Mean percentage of attached synaptic ribbons. Significance was determined by one-way ANOVA test ( P < 0.001, n = 3–4 animals). Post hoc Tukey's tests for multiple comparisons ( P < 0.001 for P30 vs. all other age groups).

    Article Snippet: Cone photoreceptor terminals were then 3D reconstructed in Imaris (BitPlane, Zurich, Switzerland), and ribbon or α1f labelling outside of the reconstructed cone terminal was subtracted.

    Techniques:

    Correlations of the synaptic parameters during development. Data points are the measured synaptic parameters in the examined age groups (P6–P7, P8–P9, P10–P11, P12–P13, and >P30). The standard error of the mean of the x axis and y axis variables are included on all plots. All correlation tests were performed using the Pearson's correlation test. ( A – C ) Cone photoreceptor terminal area plotted against different synaptic parameters. Number of synaptic ribbons ( A ) ( P < 0.001, r = 0.993), I Ca (B, P = 0.009, r = 0.960), and AZ area per cone photoreceptor terminal ( C ) ( P = 0.008, r = 0.965). ( D ) Plot of I Ca against the number of AZs per cone photoreceptor terminal ( P = 0.0307, r = 0.912). ( E 1 – 2 ) Plot of tonic SV release per cone photoreceptor against different synaptic parameters. I Ca ( E 1 ) ( P = 0.032, r = 0.911), number of AZs per cone photoreceptor terminal ( E 2 ) ( P = 0.003, r = 0.983). ( F 1 – 2 ) Plot of spontaneous SV release per cone photoreceptor against different synaptic parameters. I Ca ( F 1 ) ( P = 0.377, r = 0.512), number of AZs per cone photoreceptor terminal ( F 2 ) ( P = 0.468, r = 0.432). ( G ) Plot of multiquantal charge transfer against number of attached synaptic ribbons ( P = 0.00168, r = 0.987). ( H 1 ) Plot of number of attached synaptic ribbons per cone terminal against tonic SV release ( black ) and I ca amplitude per AZ ( red ). ( H 2 ) Plot of tonic SV release against attached ribbon content per cone.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Functional and Structural Development of Mouse Cone Photoreceptor Ribbon Synapses

    doi: 10.1167/iovs.63.3.21

    Figure Lengend Snippet: Correlations of the synaptic parameters during development. Data points are the measured synaptic parameters in the examined age groups (P6–P7, P8–P9, P10–P11, P12–P13, and >P30). The standard error of the mean of the x axis and y axis variables are included on all plots. All correlation tests were performed using the Pearson's correlation test. ( A – C ) Cone photoreceptor terminal area plotted against different synaptic parameters. Number of synaptic ribbons ( A ) ( P < 0.001, r = 0.993), I Ca (B, P = 0.009, r = 0.960), and AZ area per cone photoreceptor terminal ( C ) ( P = 0.008, r = 0.965). ( D ) Plot of I Ca against the number of AZs per cone photoreceptor terminal ( P = 0.0307, r = 0.912). ( E 1 – 2 ) Plot of tonic SV release per cone photoreceptor against different synaptic parameters. I Ca ( E 1 ) ( P = 0.032, r = 0.911), number of AZs per cone photoreceptor terminal ( E 2 ) ( P = 0.003, r = 0.983). ( F 1 – 2 ) Plot of spontaneous SV release per cone photoreceptor against different synaptic parameters. I Ca ( F 1 ) ( P = 0.377, r = 0.512), number of AZs per cone photoreceptor terminal ( F 2 ) ( P = 0.468, r = 0.432). ( G ) Plot of multiquantal charge transfer against number of attached synaptic ribbons ( P = 0.00168, r = 0.987). ( H 1 ) Plot of number of attached synaptic ribbons per cone terminal against tonic SV release ( black ) and I ca amplitude per AZ ( red ). ( H 2 ) Plot of tonic SV release against attached ribbon content per cone.

    Article Snippet: Cone photoreceptor terminals were then 3D reconstructed in Imaris (BitPlane, Zurich, Switzerland), and ribbon or α1f labelling outside of the reconstructed cone terminal was subtracted.

    Techniques: